Standard PCR
5 steps · PCR
Master mix assembly, thermal cycling, and a gel check.
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5 steps · PCR
Master mix assembly, thermal cycling, and a gel check.
6 steps · Biofilm
Static biofilm growth with a crystal violet readout.
5 steps · Enumeration
Ten-fold serial dilution series spotted for CFU counting.
4 steps · Media prep
Weigh, dissolve, adjust pH, and sterilise a batch of medium.
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Toyomasa Katagiri · https://www.protocols.io/view/05-peptide-preparation-for-ip-ms-without-fractiona-hc46b2yzf · CC-BY-4.0
Joseph Mosquera, Lieselot Carrette, Olivier George · https://www.protocols.io/view/adipoclear-rat-brain-hemisphere-hbu9b2nz7 · CC-BY-4.0
Renpei Kato · https://www.protocols.io/view/13-doxorubicin-induced-cell-death-assay-in-3d-sphe-hbyhb2pt7 · CC-BY-4.0
Toyomasa Katagiri · https://www.protocols.io/view/06-peptide-preparation-for-ip-ms-mitochondrial-fra-hc47b2yzp · CC-BY-4.0
Renpei Kato · https://www.protocols.io/view/07-mitosox-based-ros-analysis-in-caki1-cells-follo-hbx8b2prx · CC-BY-4.0
Renpei Kato · https://www.protocols.io/view/12-protein-expression-after-h-o-treatment-in-hek29-hbynb2pvf · CC-BY-4.0
Renpei Kato · https://www.protocols.io/view/14-protein-expression-after-mitochondrial-ros-scav-hbyjb2pup · CC-BY-4.0
Toyomasa Katagiri · https://www.protocols.io/view/04-peptide-preparation-for-lc-ms-without-ip-hc45b2yy7 · CC-BY-4.0